Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

Brain Res. 2002 Dec 27;958(2):305-11. doi: 10.1016/s0006-8993(02)03604-1.

Abstract

Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 microM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Hypoxia / drug effects
  • Cell Hypoxia / physiology
  • Enzyme Inhibitors / pharmacology
  • Erythropoietin / biosynthesis
  • Erythropoietin / pharmacology*
  • Hippocampus / drug effects*
  • Hippocampus / metabolism
  • Hippocampus / physiology
  • In Vitro Techniques
  • Janus Kinase 2
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins*
  • Rats
  • Rats, Wistar
  • Receptors, Erythropoietin / biosynthesis
  • Synaptic Transmission / drug effects*
  • Synaptic Transmission / physiology

Substances

  • Enzyme Inhibitors
  • Proto-Oncogene Proteins
  • Receptors, Erythropoietin
  • Erythropoietin
  • Protein-Tyrosine Kinases
  • Jak2 protein, rat
  • Janus Kinase 2