Abstract
Plexins belonging to the plexin-A subfamily form complexes with neuropilins and propagate signals of class 3 semaphorins into neurons, even though they do not directly bind the semaphorins. In this study, we identified a new member of the plexin-A subfamily in the mice, plexin-A4, and showed that it was expressed in the developing nervous system with a pattern different to that of other members of the plexin-A subfamily (plexin-A1, plexin-A2 and plexin-A3). COS-7 cells coexpressing plexin-A4 with neuropilin-1 were induced to contract by Sema3A, a member of the class 3 semaphorin. Ectopic expression of plexin-A4 in mitral cells that are originally insensitive to Sema3A resulted in the collapse of growth cones in the presence of Sema3A. These results suggest that plexin-A4 plays a role in the propagation of Sema3A activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Axons / metabolism
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Brain / embryology
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Brain / metabolism
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COS Cells
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cloning, Molecular
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Ganglia / embryology
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Ganglia, Sensory / embryology
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Gene Expression Regulation, Developmental*
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Growth Cones / metabolism
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Mice
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Mice, Inbred ICR
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Molecular Sequence Data
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Multigene Family
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Mutation
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Nervous System / embryology*
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Nervous System / growth & development
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Neuropilin-1 / genetics
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Neuropilin-1 / metabolism
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Olfactory Bulb / cytology
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Olfactory Bulb / embryology
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Organ of Corti / growth & development
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Retina / growth & development
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Semaphorin-3A*
Substances
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Carrier Proteins
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Nerve Tissue Proteins
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Plxna1 protein, mouse
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Plxna4 protein, mouse
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Receptors, Cell Surface
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Sema3a protein, mouse
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Semaphorin-3A
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Neuropilin-1