Abstract
Neuronal connectivity is established by the axo-dendritic polarity, correct guidance and targeting of neurons. Unlike for axons, the mechanisms responsible for directed outgrowth of dendrites are not well understood. Using single-cell labeling, we describe specific guidance defects in dendrites of identified neurons in frazzled, robo, netrin and commissureless mutant embryos of Drosophila melanogaster. We found that the cell-surface molecules Frazzled and Robo work as guidance molecules not only for axons but also for dendrites as they navigate within the CNS. Furthermore, we report that each neuron showed a cell-autonomous and independent use of guidance molecules.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Axons / metabolism
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Central Nervous System / cytology
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Central Nervous System / embryology*
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Central Nervous System / metabolism*
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Dendrites / metabolism*
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Drosophila Proteins*
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Drosophila melanogaster
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Embryo, Nonmammalian / innervation
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Fluorescent Dyes
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Membrane Proteins / deficiency
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Motor Neurons / cytology
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Motor Neurons / metabolism
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Mutation
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Nerve Growth Factors / deficiency
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Nerve Growth Factors / genetics
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Nerve Tissue Proteins
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Netrin Receptors
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Netrin-1
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Netrins
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Receptors, Cell Surface / deficiency
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism*
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Receptors, Immunologic / deficiency
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism*
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Roundabout Proteins
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Tumor Suppressor Proteins
Substances
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Drosophila Proteins
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Fluorescent Dyes
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Membrane Proteins
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Nerve Growth Factors
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Nerve Tissue Proteins
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Netrin Receptors
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Netrins
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Receptors, Cell Surface
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Receptors, Immunologic
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Tumor Suppressor Proteins
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comm protein, Drosophila
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fra protein, Drosophila
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Netrin-1
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netrin-2