A family of metabotropic glutamate receptors

Neuron. 1992 Jan;8(1):169-79. doi: 10.1016/0896-6273(92)90118-w.

Abstract

Three cDNA clones, mGluR2, mGluR3, and mGluR4, were isolated from a rat brain cDNA library by cross-hybridization with the cDNA for a metabotropic glutamate receptor (mGluR1). The cloned receptors show considerable sequence similarity with mGluR1 and possess a large extracellular domain preceding the seven putative membrane-spanning segments. mGluR2 is expressed in some particular neuronal cells different from those expressing mGluR1 and mediates an efficient inhibition of forskolin-stimulated cAMP formation in cDNA-transfected cells. The mGluRs thus form a novel family of G protein-coupled receptors that differ in their signal transduction and expression patterns.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry
  • CHO Cells
  • Cell Membrane / chemistry
  • Cerebellum / chemistry
  • Cerebral Cortex / chemistry
  • Cloning, Molecular
  • Colforsin / pharmacology
  • Cricetinae
  • Cyclic AMP / biosynthesis
  • DNA / chemistry
  • DNA / isolation & purification
  • Gene Expression
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Olfactory Bulb / chemistry
  • RNA, Messenger / analysis
  • Rats
  • Receptors, Glutamate
  • Receptors, Neurotransmitter / chemistry
  • Receptors, Neurotransmitter / genetics*
  • Signal Transduction
  • Tissue Distribution
  • Transfection

Substances

  • RNA, Messenger
  • Receptors, Glutamate
  • Receptors, Neurotransmitter
  • Colforsin
  • DNA
  • Cyclic AMP