Abstract
The basis for differences in activity-dependent trafficking of AMPA receptors (AMPARs) and NMDA receptors (NMDARs) remains unclear. Using single-molecule tracking, we found different lateral mobilities for AMPARs and NMDARs: changes in neuronal activity modified AMPAR but not NMDAR mobility, whereas protein kinase C activation modified both. Differences in mobility were mainly detected for extrasynaptic AMPARs, suggesting that receptor diffusion between synaptic and extrasynaptic domains is involved in plasticity processes.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Animals, Newborn
-
Antibodies
-
Carbocyanines / pharmacokinetics
-
Cells, Cultured
-
Hippocampus / cytology*
-
Neurons / metabolism*
-
Potassium Chloride / pharmacology
-
Protein Kinase C / antagonists & inhibitors
-
Protein Transport / physiology
-
Rats
-
Receptors, AMPA / metabolism*
-
Receptors, N-Methyl-D-Aspartate / metabolism*
-
Statistics, Nonparametric
-
Synapses / metabolism
-
Tetradecanoylphorbol Acetate / pharmacology
-
Tetrodotoxin / pharmacology
-
Time Factors
Substances
-
Antibodies
-
Carbocyanines
-
NR1 NMDA receptor
-
Receptors, AMPA
-
Receptors, N-Methyl-D-Aspartate
-
cyanine dye 3
-
Tetrodotoxin
-
Potassium Chloride
-
Protein Kinase C
-
Tetradecanoylphorbol Acetate
-
glutamate receptor ionotropic, AMPA 2