Abstract
Neuronal migrations along glial fibers provide a primary pathway for the formation of cortical laminae. To examine the mechanisms underlying glial-guided migration, we analyzed the dynamics of cytoskeletal and signaling components in living neurons. Migration involves the coordinated two-stroke movement of a perinuclear tubulin 'cage' and the centrosome, with the centrosome moving forward before nuclear translocation. Overexpression of mPar6alpha disrupts the perinuclear tubulin cage, retargets PKCzeta and gamma-tubulin away from the centrosome, and inhibits centrosomal motion and neuronal migration. Thus, we propose that during neuronal migration the centrosome acts to coordinate cytoskeletal dynamics in response to mPar6alpha-mediated signaling.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Astrocytes / physiology*
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Cell Movement / drug effects
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Cell Movement / physiology*
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Cell Nucleus / metabolism
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Cell Polarity / physiology
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Cell Surface Extensions / drug effects
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Cell Surface Extensions / physiology
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Cells, Cultured
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Centrosome / metabolism
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Cerebellum / cytology*
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Cloning, Molecular / methods
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Coculture Techniques / methods
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Diagnostic Imaging / methods
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Dynactin Complex
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Genetic Vectors / physiology
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Glial Fibrillary Acidic Protein / metabolism
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Green Fluorescent Proteins / metabolism
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Immunohistochemistry / methods
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Mice
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Microtubule-Associated Proteins / metabolism
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Neurons / cytology
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Neurons / drug effects
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Neurons / physiology*
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Neurons / virology
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Organ Culture Techniques
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Photobleaching
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Protein Kinase C / metabolism
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Protein Kinase C-epsilon
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Proteins / genetics
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Proteins / metabolism
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Proteins / physiology*
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RNA, Catalytic / pharmacology
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Signal Transduction / physiology*
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Time Factors
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Transfection / methods
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Tubulin / metabolism
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Videotape Recording / methods
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Zidovudine / metabolism
Substances
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Dynactin Complex
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Glial Fibrillary Acidic Protein
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Microtubule-Associated Proteins
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Proteins
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RNA, Catalytic
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Tubulin
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hairpin ribozyme
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Green Fluorescent Proteins
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Zidovudine
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Prkce protein, mouse
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Protein Kinase C
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Protein Kinase C-epsilon