Sumoylation of the SOX10 transcription factor regulates its transcriptional activity

FEBS Lett. 2006 Mar 6;580(6):1635-41. doi: 10.1016/j.febslet.2006.02.011. Epub 2006 Feb 17.

Abstract

SRY-related HMG box-containing factor 10 (SOX10) is a transcription factor essential for neural crest development and differentiation, and involved in Waardenburg syndrome type IV and PCWH syndrome. Here we show that the SOX10 protein is modified by sumoylation, a highly dynamic post-translational modification that affects stability, activity and localisation of some specific transcription factors. Three sumoylation consensus sites were found in the SOX10 protein, all of them are functional and modulate SOX10 activity. Sumoylation does not affect SOX10 sub-cellular localisation, but represses its transcriptional activity on two of its target genes, GJB1 and MITF, and modulates its synergy with its cofactors EGR2 and PAX3 on these promoters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Nucleus / chemistry
  • Connexins / genetics
  • Consensus Sequence
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Early Growth Response Protein 2 / metabolism
  • Gap Junction beta-1 Protein
  • Gene Expression Regulation
  • HeLa Cells
  • High Mobility Group Proteins / analysis
  • High Mobility Group Proteins / genetics
  • High Mobility Group Proteins / metabolism*
  • Humans
  • Microphthalmia-Associated Transcription Factor / genetics
  • Molecular Sequence Data
  • PAX3 Transcription Factor
  • Paired Box Transcription Factors / metabolism
  • Promoter Regions, Genetic
  • Protein Processing, Post-Translational*
  • SOXE Transcription Factors
  • Transcription Factors / analysis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Ubiquitin-Conjugating Enzymes / metabolism*

Substances

  • Connexins
  • DNA-Binding Proteins
  • EGR2 protein, human
  • Early Growth Response Protein 2
  • High Mobility Group Proteins
  • Microphthalmia-Associated Transcription Factor
  • PAX3 Transcription Factor
  • PAX3 protein, human
  • Paired Box Transcription Factors
  • SOX10 protein, human
  • SOXE Transcription Factors
  • Transcription Factors
  • Ubiquitin-Conjugating Enzymes
  • ubiquitin-conjugating enzyme UBC9