Abstract
192 IgG, a monoclonal antibody to the rat nerve growth factor (NGF) receptor, was disulfide-coupled to saporin, a ribosome-inactivating protein. Systemic injection of 192 IgG-saporin destroyed sympathetic postganglionic neurons and some sensory neurons. Injection of 192 IgG-saporin into the lateral ventricle destroyed cholinergic neurons of the basal forebrain. These results show that antineuronal immunotoxins are a powerful approach that may prove useful in a variety of neurobiological applications.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Cell Survival / drug effects
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Functional Laterality
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Ganglia, Sympathetic / drug effects
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Ganglia, Sympathetic / pathology*
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Immunoenzyme Techniques
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Immunoglobulin G / analysis
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Immunotoxins / analysis
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Immunotoxins / toxicity*
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N-Glycosyl Hydrolases*
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Nerve Growth Factors / metabolism
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Neurons / drug effects
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Neurons / pathology*
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Neurons, Afferent / drug effects
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Neurons, Afferent / pathology
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Plant Proteins / toxicity*
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Rats
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Receptors, Cell Surface / immunology*
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Receptors, Nerve Growth Factor
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Ribosome Inactivating Proteins, Type 1
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Saporins
Substances
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Antibodies, Monoclonal
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Immunoglobulin G
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Immunotoxins
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Nerve Growth Factors
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Plant Proteins
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Receptors, Cell Surface
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Receptors, Nerve Growth Factor
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Ribosome Inactivating Proteins, Type 1
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N-Glycosyl Hydrolases
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Saporins