Abstract
Neurogranin is capable of regulating protein kinase C and Ca2+/calmodulin-dependent protein kinase II. In this study, we examined the role of neurogranin in opioid tolerance. Increased phosphorylation of neurogranin was found in opioid tolerance. Opioid tolerance was absent in morphine (100 mg/kg)-treated mice that were also pretreated with neurogranin antisense oligodeoxynucleotides (2 microg/day, i.c.v. for 3 days). The behavioral effect correlated with the decreased expression of neurogranin. These data suggest that neurogranin may be critical in the development of opioid tolerance.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Analgesics, Opioid / administration & dosage
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Analgesics, Opioid / antagonists & inhibitors
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Analgesics, Opioid / pharmacology
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Analysis of Variance
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Animals
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Behavior, Animal / drug effects
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Behavior, Animal / physiology
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Drug Tolerance / physiology*
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Injections, Intraventricular
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Injections, Subcutaneous
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Male
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Mice
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Mice, Inbred ICR
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Morphine / administration & dosage
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Morphine / antagonists & inhibitors
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Morphine / pharmacology*
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Neurogranin / genetics
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Neurogranin / physiology*
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Oligonucleotides, Antisense / administration & dosage
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Oligonucleotides, Antisense / pharmacology*
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Pain Measurement / methods
Substances
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Analgesics, Opioid
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Oligonucleotides, Antisense
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Neurogranin
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Morphine