Light and electron microscopic analysis of projection neurons retrogradely labeled with Fluoro-Gold: notes on the application of antibodies to Fluoro-Gold

J Neurosci Methods. 1990 Oct;35(1):31-7. doi: 10.1016/0165-0270(90)90091-s.

Abstract

Fluoro-Gold has been a very popular fluorescent tracer used recently for retrogradely labeling projection neurons. In this study we described the advantages of using antibodies to Fluoro-Gold in conventional immunohistochemical reaction protocols to further extend the usefulness of this tracer for both light and electron microscopic neuroanatomical studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Axonal Transport
  • Brain / anatomy & histology*
  • Brain / cytology
  • Brain / enzymology
  • Choline O-Acetyltransferase / analysis
  • Fluorescent Antibody Technique
  • Fluorescent Dyes
  • Immunoenzyme Techniques
  • Immunohistochemistry / methods
  • Microscopy, Electron / methods
  • Neurons / cytology*
  • Neurons / enzymology
  • Stilbamidines*

Substances

  • 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
  • Fluorescent Dyes
  • Stilbamidines
  • Choline O-Acetyltransferase