Abstract
It has generally been believed that the diffusion limit set by the nuclear pore for protein is 60kDa. We here studied the cellular localization of several artificial proteins and found that the diffusion limit set by the nuclear pore is not as small as previously thought. The results indicate that the maximal size of protein to diffuse through the nuclear pore complex could be quite larger than 60kDa, thus greatly extending the diffusion limit that the nuclear pore can accommodate.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Active Transport, Cell Nucleus
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Cell Nucleus / metabolism*
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Diffusion
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HCT116 Cells
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HeLa Cells
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Humans
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Mitogen-Activated Protein Kinase 1 / genetics
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Mitogen-Activated Protein Kinase 1 / metabolism
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Molecular Weight
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Nuclear Pore / metabolism*
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Particle Size*
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Proteins / chemistry
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Proteins / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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Proteins
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Recombinant Fusion Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Mitogen-Activated Protein Kinase 1