Long-term expressional changes of Na+ -K+ -Cl- co-transporter 1 (NKCC1) and K+ -Cl- co-transporter 2 (KCC2) in CA1 region of hippocampus following lithium-pilocarpine induced status epilepticus (PISE)

Brain Res. 2008 Jul 24:1221:141-6. doi: 10.1016/j.brainres.2008.04.047. Epub 2008 Apr 27.

Abstract

NKCC1 and KCC2 are encoded by slc12 gene family and involved in the maintenance of intracellular chloride concentration which may be associated with epileptogenesis. In this study, we aimed to investigate the long-term expression profiles of NKCC1 and KCC2 in CA1 region in the mice model of lithium-pilocarpine induced status epilepticus (PISE) and their relationship with epileptogenesis. We found NKCC1 mRNA and proteins were up-regulated at 1 d, 14 d and 45 d after pilocarpine injection, while KCC2 was down-regulated. According to obtained results, there were some expressional changes of NKCC1 and KCC2. Deregulation of their expression may break the balance of intracellular and extracellular chloride concentration which contributes to the mechanism of hyperexcitability leading to seizures. Also it may provide new drug targets for development of new antiepileptic medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chloride Channels / drug effects
  • Chloride Channels / metabolism
  • Chlorides / metabolism
  • Disease Models, Animal
  • Epilepsy / chemically induced
  • Epilepsy / metabolism*
  • Epilepsy / physiopathology
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Hippocampus / physiopathology
  • K Cl- Cotransporters
  • Lithium Compounds / pharmacology
  • Male
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Mice
  • Mice, Inbred BALB C
  • Muscarinic Agonists / toxicity
  • Neurons / drug effects
  • Neurons / metabolism
  • Pilocarpine / toxicity
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • Sodium-Potassium-Chloride Symporters / drug effects
  • Sodium-Potassium-Chloride Symporters / genetics
  • Sodium-Potassium-Chloride Symporters / metabolism*
  • Solute Carrier Family 12, Member 2
  • Status Epilepticus / chemically induced
  • Status Epilepticus / metabolism*
  • Status Epilepticus / physiopathology
  • Symporters / drug effects
  • Symporters / genetics
  • Symporters / metabolism*
  • Time Factors
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • Chloride Channels
  • Chlorides
  • Lithium Compounds
  • Muscarinic Agonists
  • RNA, Messenger
  • Slc12a2 protein, mouse
  • Sodium-Potassium-Chloride Symporters
  • Solute Carrier Family 12, Member 2
  • Symporters
  • Pilocarpine