Biosensor plates detect mitochondrial physiological regulators and mutations in vivo

Anabela P Rolo; Carlos M Palmeira; Gino A Cortopassi

doi:10.1016/j.ab.2008.10.002

Biosensor plates detect mitochondrial physiological regulators and mutations in vivo

Anal Biochem. 2009 Feb 1;385(1):176-8. doi: 10.1016/j.ab.2008.10.002. Epub 2008 Oct 7.

Authors

Anabela P Rolo¹, Carlos M Palmeira, Gino A Cortopassi

Affiliation

¹ Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.

Abstract

The measurement of mitochondrial activity in living cells is usually not straightforward, even though it is quite important in physiological and pathophysiological processes. We describe a high-throughput method for measurement of mitochondrial oxygen consumption in living cells, based on the Becton-Dickinson Biosensor plates.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology
Dose-Response Relationship, Drug
Humans
Mitochondrial Proteins / analysis*
Mitochondrial Proteins / genetics*
Mitochondrial Proteins / metabolism
Oxygen Consumption
Point Mutation / genetics*
Time Factors
Tumor Cells, Cultured

Substances

Mitochondrial Proteins
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone

Abstract

Publication types

MeSH terms

Substances

Grants and funding