The paraventricular nucleus of the hypothalamus (PVH) coordinates neuroendocrine, autonomic, and behavioral responses to help maintain energy and body water balance. The rat paraventricular nucleus has three major divisions: descending with axonal projections to somatomotor-behavioral and autonomic circuitry, magnocellular neuroendocrine with projections directly to the posterior pituitary, and parvicellular neuroendocrine with projections to the median eminence for controlling anterior pituitary hormone secretion. The present work was undertaken to provide high-resolution mapping of spatial relationships among the two magnocellular neuroendocrine and five parvicellular neuroendocrine neuron types throughout the nucleus. Double immunohistochemical labeling for two neuron types combined with retrograde labeling to identify neuroendocrine neurons positively was used in individual sections spaced 45 mum apart, along with a grid transfer method for reducing plane of section artifacts when comparing staining pattern data between animals. The results indicate that whereas each neuroendocrine neuron phenotype displays a unique distribution pattern, there is extensive partial overlap in a complex pattern between small "hot spots" with a relatively high density of a particular neuron type and few if any other phenotypes. In addition, the distribution of non-neuroendocrine neurons staining with each of the markers (but not retrogradely labeled) was mapped and compared with each other and with the neuroendocrine neuron populations. This spatial organization raises important questions about the differential functional regulation of individual-and perhaps sets of-neuroendocrine motor neuron populations in the PVH by synaptic mechanisms and by less traditional mechanisms like dendritic neurotransmitter release and gap junctions within and between neuron types.