Abstract
Numerous studies have shown that biochemical and behavioral effects of cocaine are mediated by dopamine D1 receptor (D1R) and NMDA R1 receptor (NR1)-mediated transmission. In this study, we investigated the physical interactions between D1R and NR1 in response to acute cocaine administration in a time course of 5-60 min. In the caudate-putamen (CPu) of male Fischer rats, a single cocaine injection (30 mg/kg) reduced D1R-NR1 protein-protein interactions 30 min after treatment. In addition, activation or blockade of the NMDA receptor using NMDA (25mg/kg) or MK-801 (0.25mg/kg), respectively, also reduced the D1R-NR1 physical interactions. Acute cocaine administration did not alter total D1R or NR1 protein levels in our time course of study. These results indicate that D1R-NR1 physical interaction rather than total protein levels may regulate the intracellular signaling after acute cocaine administration.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Blotting, Western
-
Caudate Nucleus / drug effects*
-
Caudate Nucleus / metabolism
-
Cocaine / pharmacology*
-
Dizocilpine Maleate / pharmacology
-
Dopamine Uptake Inhibitors / pharmacology*
-
Excitatory Amino Acid Agonists / pharmacology
-
Excitatory Amino Acid Antagonists / pharmacology
-
Immunoprecipitation
-
Male
-
N-Methylaspartate / pharmacology
-
Putamen / drug effects*
-
Putamen / metabolism
-
Rats
-
Rats, Inbred F344
-
Receptors, Dopamine D1 / metabolism*
-
Receptors, N-Methyl-D-Aspartate / agonists
-
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
-
Receptors, N-Methyl-D-Aspartate / metabolism*
-
Time Factors
-
Tubulin / metabolism
Substances
-
Dopamine Uptake Inhibitors
-
Excitatory Amino Acid Agonists
-
Excitatory Amino Acid Antagonists
-
NR1 NMDA receptor
-
Receptors, Dopamine D1
-
Receptors, N-Methyl-D-Aspartate
-
Tubulin
-
N-Methylaspartate
-
Dizocilpine Maleate
-
Cocaine