Differential expression of the mouse D2 dopamine receptor isoforms

FEBS Lett. 1991 Jan 28;278(2):239-43. doi: 10.1016/0014-5793(91)80125-m.

Abstract

We have identified and characterized the cDNAs corresponding to the mouse D2 dopamine receptors. We show that in the mouse the D2 dopamine receptor is found in two forms, generated by alternative splicing of the same gene, mRNA distribution analysis of areas expressing the D2 receptors shows that the larger form is the most abundant, except in the brain stem where the shorter form is predominant. Membranes of mammalian cells transiently transfected with both forms of D2 receptor bind [3H]spiperone with a high affinity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Brain / physiology*
  • Brain Mapping
  • Bromocriptine / metabolism
  • Cloning, Molecular
  • DNA / genetics
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Receptors, Dopamine / genetics*
  • Receptors, Dopamine / metabolism
  • Recombinant Proteins / metabolism
  • Spiperone / metabolism

Substances

  • RNA, Messenger
  • Receptors, Dopamine
  • Recombinant Proteins
  • Bromocriptine
  • Spiperone
  • DNA

Associated data

  • GENBANK/X55674
  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X62840
  • GENBANK/X62841