High-throughput in vivo vertebrate screening

Carlos Pardo-Martin; Tsung-Yao Chang; Bryan Kyo Koo; Cody L Gilleland; Steven C Wasserman; Mehmet Fatih Yanik

doi:10.1038/nmeth.1481

High-throughput in vivo vertebrate screening

Nat Methods. 2010 Aug;7(8):634-6. doi: 10.1038/nmeth.1481. Epub 2010 Jul 18.

Authors

Carlos Pardo-Martin¹, Tsung-Yao Chang, Bryan Kyo Koo, Cody L Gilleland, Steven C Wasserman, Mehmet Fatih Yanik

Affiliation

¹ Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.

Abstract

We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Automation
Axons*
Embryo, Nonmammalian
Equipment Design
Laser Therapy
Mass Screening / methods*
Microscopy, Confocal / instrumentation*
Models, Animal
Mutation
Retina / cytology*
Zebrafish

Abstract

Publication types

MeSH terms

Grants and funding