Abstract
Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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DNA / genetics*
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DNA / isolation & purification
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Dinoprostone / biosynthesis
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Escherichia coli / genetics
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Fibroblasts / metabolism
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Gene Expression*
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Glycosylation
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Humans
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Interleukin 1 Receptor Antagonist Protein
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Interleukin-1 / genetics
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Interleukin-1 / pharmacology
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Molecular Sequence Data
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Monocytes / metabolism
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Nucleic Acid Hybridization
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Proteins / genetics*
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Proteins / pharmacology
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Receptors, Immunologic / antagonists & inhibitors*
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Receptors, Interleukin-1
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Sequence Homology, Nucleic Acid
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Sialoglycoproteins*
Substances
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IL1RN protein, human
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Interleukin 1 Receptor Antagonist Protein
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Interleukin-1
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Proteins
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Interleukin-1
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Recombinant Proteins
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Sialoglycoproteins
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DNA
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Dinoprostone