Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist

Nature. 1990 Jan 25;343(6256):341-6. doi: 10.1038/343341a0.

Abstract

Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / genetics*
  • DNA / isolation & purification
  • Dinoprostone / biosynthesis
  • Escherichia coli / genetics
  • Fibroblasts / metabolism
  • Gene Expression*
  • Glycosylation
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / genetics
  • Interleukin-1 / pharmacology
  • Molecular Sequence Data
  • Monocytes / metabolism
  • Nucleic Acid Hybridization
  • Proteins / genetics*
  • Proteins / pharmacology
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Receptors, Immunologic / antagonists & inhibitors*
  • Receptors, Interleukin-1
  • Recombinant Proteins / genetics
  • Recombinant Proteins / pharmacology
  • Sequence Homology, Nucleic Acid
  • Sialoglycoproteins*

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Proteins
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Sialoglycoproteins
  • DNA
  • Dinoprostone