In cerebellar sections of the feline brain processed for choline acetyltransferase-like immunoreactivity, a population of distinctly stained cells was discovered in the granular layer of the cortex in both vermis and hemispheres. Their position and morphology qualify them as Golgi cells, but their density indicated that they comprise less than 5% of all cerebellar Golgi cells. Varicose immunoreactive fiber nets in all cortical layers also contribute to seemingly widespread cholinergic systems in the cerebellar cortex.