Abstract
We studied cultured hippocampal neurons from embryonic wildtype, major histocompatibility complex class I (MHCI) heavy chain-deficient (K(b)D(b)-/-) and NSE-D(b) (which have elevated neuronal MHCI expression) C57BL/6 mice. K(b)D(b)-/- neurons displayed slower neuritogenesis and establishment of polarity, while NSE-D(b) neurons had faster neurite outgrowth, more primary neurites, and tended to have accelerated polarization. Additional studies with ß2M-/- neurons, exogenous ß2M, and a self-MHCI monomer suggest that free heavy chain cis interactions with other surface molecules can promote neuritogenesis while tripartite MHCI interactions with classical MHCI receptors can inhibit axon outgrowth. Together with the results of others, MHCI appears to differentially modulate neuritogenesis and synaptogenesis.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Actins / metabolism
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Analysis of Variance
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Animals
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Axons / physiology
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Cell Polarity / drug effects
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Cell Polarity / physiology*
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Cells, Cultured
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Embryo, Mammalian
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Developmental / physiology
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Hippocampus / cytology
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Histocompatibility Antigens Class I / genetics
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Histocompatibility Antigens Class I / metabolism*
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Histocompatibility Antigens Class I / pharmacology
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Microtubule-Associated Proteins / metabolism
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Neurites / drug effects
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Neurites / physiology
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Neurogenesis / physiology*
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Neurons / drug effects
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Neurons / physiology*
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Phosphopyruvate Hydratase / genetics
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Recombinant Proteins / pharmacology
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Time Factors
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Tubulin / metabolism
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beta 2-Microglobulin / deficiency
Substances
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Actins
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Histocompatibility Antigens Class I
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Microtubule-Associated Proteins
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Mtap2 protein, mouse
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Recombinant Proteins
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Tubulin
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beta 2-Microglobulin
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beta3 tubulin, mouse
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Phosphopyruvate Hydratase