Abstract
G protein-coupled receptors (GPCRs) affect many physiological processes by modulating both intrinsic membrane conductances and synaptic transmission. This study describes spontaneous miniature inhibitory postsynaptic currents mediated by vesicular dopamine release acting locally on metabotropic D2 receptors leading to the activation of a G protein-coupled inwardly rectifying potassium conductance. Thus, individual exocytotic events result in spontaneous GPCR-mediated transmission, similar to synaptic activation of classical ligand-gated ion channels.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Analysis of Variance
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Animals
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Chelating Agents / pharmacology
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Colforsin / pharmacology
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Dopamine / metabolism
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Dopamine Agents / pharmacology
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Egtazic Acid / analogs & derivatives
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Egtazic Acid / pharmacology
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Female
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GABA Antagonists / pharmacology
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Humans
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In Vitro Techniques
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Inhibitory Postsynaptic Potentials / drug effects
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Inhibitory Postsynaptic Potentials / genetics
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Inhibitory Postsynaptic Potentials / physiology*
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Levodopa / pharmacology
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Male
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Mesencephalon / cytology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Transgenic
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Neurons / drug effects
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Neurons / physiology*
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Organophosphorus Compounds / pharmacology
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Patch-Clamp Techniques
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Receptors, Dopamine D2 / genetics
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Receptors, G-Protein-Coupled / metabolism*
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Serotonin Agents / pharmacology
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Tyrosine 3-Monooxygenase / genetics
Substances
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Chelating Agents
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DRD2 protein, human
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Dopamine Agents
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GABA Antagonists
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Organophosphorus Compounds
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Receptors, Dopamine D2
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Receptors, G-Protein-Coupled
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Serotonin Agents
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Colforsin
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Levodopa
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Egtazic Acid
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CGP 35348
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Tyrosine 3-Monooxygenase
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1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
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Dopamine