Abstract
We have isolated a complementary DNA clone by screening a rat brain cDNA library for expression of kainate-gated ion channels in Xenopus oocytes. The cDNA encodes a single protein of relative molecular mass (Mr) 99,800 which on expression in oocytes forms a functional ion channel possessing the electrophysiological and pharmacological properties of the kainate subtype of the glutamate receptor family in the mammalian central nervous system.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Aspartic Acid / analogs & derivatives
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Aspartic Acid / pharmacology
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Base Sequence
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Brain Chemistry
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Cloning, Molecular*
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DNA / genetics
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DNA / isolation & purification
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Electrophysiology
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Gene Expression
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Glutamates / pharmacology
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Glutamic Acid
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Ion Channels / drug effects
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Ion Channels / physiology
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Kainic Acid / metabolism
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Kainic Acid / pharmacology
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Molecular Sequence Data
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Molecular Weight
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N-Methylaspartate
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Oocytes / metabolism
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Plasmids
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RNA / analysis
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Rats
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Receptors, Glutamate
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Receptors, Neurotransmitter / genetics*
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Tissue Distribution
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Transcription, Genetic
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Transfection
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Xenopus
Substances
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Glutamates
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Ion Channels
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Receptors, Glutamate
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Receptors, Neurotransmitter
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Aspartic Acid
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Glutamic Acid
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RNA
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N-Methylaspartate
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DNA
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Kainic Acid