In the present investigation it was shown that electrical or chemical (D,L-homocysteic acid, DLH) stimulation in a defined area of the medial prefrontal cortex inhibits cardiovascular components of the defence reaction elicited by stimulation in the basal nucleus of the amygdala or in the hypothalamus in rats anaesthetized by Saffan. Electrical stimulation in the dorsal part of the nucleus accumbens or ventral part of the nucleus caudate had the same effect, while chemical stimulation (DLH) in these areas was not effective. In unanaesthetized rats stimulation in the prefrontal cortex or the nucleus accumbens inhibited cardiovascular and motor components of the defence reaction induced from the amygdala or hypothalamus. Stimulation in the described areas of the medial prefrontal cortex or nucleus accumbens does not induce general inhibition of motor activity since it did not affect operant, appetitive bar pressing. It is therefore concluded that the inhibition is selectively addressed to the motor activities associated with the defence reaction. It is suggested that inhibition of the cardiovascular components of the defence reaction must occur below hypothalamic level. The inhibition is most likely presynaptic since stimulation in the prefrontal cortex or nucleus accumbens alone did not produce any cardiovascular changes. It is unlikely that the efferent pathway originating in the prefrontal inhibitory area relays in the nucleus accumbens since microinjection of DLH into this nucleus was ineffective. Stimulation in "sympatho-inhibitory' areas (anterior hypothalamus, anterior cingulum) or in the nucleus raphe obscurus had no inhibitory effect on the cardiovascular components of the defence reaction.