Abstract
Polypeptides of retinal ganglion cell axons of the goldfish, regenerating in culture, were labeled by [35S]methionine after decentralization from the explant. Microscopic samples, composed of isolated axonal fields, were analyzed by SDS ultramicroelectrophoresis and autoradiography. Of the several proteins exhibiting cycloheximide-sensitive labeling, beta-tubulin and actin were consistently and prominently labeled, although the possibility of a labeled alpha-isoform with a lower mol. wt. could not be ruled out.
Publication types
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Actins / metabolism*
-
Animals
-
Cells, Cultured
-
Cycloheximide / pharmacology*
-
Cyprinidae / metabolism*
-
Goldfish / metabolism*
-
Methionine / metabolism
-
Nerve Regeneration
-
Nerve Tissue Proteins / metabolism*
-
Retina / metabolism*
-
Retinal Ganglion Cells / cytology
-
Retinal Ganglion Cells / drug effects
-
Retinal Ganglion Cells / metabolism*
-
Tubulin / metabolism*
Substances
-
Actins
-
Nerve Tissue Proteins
-
Tubulin
-
Cycloheximide
-
Methionine