Poly(ADP-ribose) catabolism in mammalian cells exposed to DNA-damaging agents

R Alvarez-Gonzalez; F R Althaus

doi:10.1016/0921-8777(89)90012-8

Poly(ADP-ribose) catabolism in mammalian cells exposed to DNA-damaging agents

Mutat Res. 1989 Sep;218(2):67-74. doi: 10.1016/0921-8777(89)90012-8.

Authors

R Alvarez-Gonzalez¹, F R Althaus

Affiliation

¹ University of Zürich-Tierspital, Institute of Pharmacology and Biochemistry, Switzerland.

PMID: 2770765
DOI: 10.1016/0921-8777(89)90012-8

Abstract

DNA damage inflicted by the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine, or by UV254nm, stimulated the catabolism of protein-bound poly(ADP-ribose) in the chromatin of cultured hepatocytes. The stimulation was highest at the largest doses of DNA-damaging treatment. As a consequence, the half-life of ADP-ribosyl polymers may drop to less than 41 s. This rapid turnover contrasts with the slow catabolism of a constitutive fraction of polymers exhibiting a half-life of 7.7 h. Our data suggest that post-incisional stimulation of poly(ADP-ribose) biosynthesis in DNA-excision repair is coupled with an adaptation of poly(ADP-ribose) catabolism in mammalian cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzamides / pharmacology*
Cells, Cultured
Chromatin / drug effects
Chromatin / metabolism
Chromatin / radiation effects
DNA Damage*
Kinetics
Liver / drug effects*
Liver / metabolism
Male
Methylnitronitrosoguanidine / pharmacology*
Nucleoside Diphosphate Sugars / metabolism*
Poly Adenosine Diphosphate Ribose / metabolism*
Poly Adenosine Diphosphate Ribose / radiation effects
Radiation-Sensitizing Agents / pharmacology
Rats
Rats, Inbred Strains
Ultraviolet Rays*

Substances

Benzamides
Chromatin
Nucleoside Diphosphate Sugars
Radiation-Sensitizing Agents
Methylnitronitrosoguanidine
Poly Adenosine Diphosphate Ribose
3-aminobenzamide