Abstract
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
MeSH terms
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Carboxy-Lyases / genetics*
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Drug Resistance, Microbial
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Fungal Proteins / genetics*
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Microbiological Techniques
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Orotic Acid / analogs & derivatives*
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Orotic Acid / pharmacology
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Orotidine-5'-Phosphate Decarboxylase / genetics*
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
Substances
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Fungal Proteins
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Orotic Acid
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5-fluoroorotic acid
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Carboxy-Lyases
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Orotidine-5'-Phosphate Decarboxylase