Abstract
Guanylyl cyclase-activating protein (GCAP) is thought to mediate Ca(2+)-sensitive regulation of guanylyl cyclase (GC), a key event in recovery of the dark state of rod photoreceptors following light exposure. Here, we characterize GCAP from several vertebrate species by molecular cloning and provide evidence that GCAP contains a heterogeneously acylated N-terminal region that interacts with GC. Vertebrate GCAPs consist of 201-205 amino acids, and sequence analysis indicates the presence fo three EF hand Ca(2+)-binding motifs. These results establish that GCAP is a novel photoreceptor-specific member of a large family of Ca(2+)-binding proteins and suggest that it participates in the Ca(2+)-binding proteins and suggest that it participates in the Ca(2+)-sensitive activation of GC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Oxidoreductases / genetics*
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology
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Base Sequence
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Calcium-Binding Proteins / chemistry
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Calcium-Binding Proteins / genetics*
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Calmodulin / chemistry
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Cattle
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Cloning, Molecular
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DNA Probes / chemistry
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Enzyme Activation
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Eye Proteins*
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Guanylate Cyclase / metabolism*
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Hippocalcin
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Humans
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Lipoproteins*
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins / chemistry
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Neurocalcin
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Nitric Oxide Synthase
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Peptide Fragments / chemistry
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Photoreceptor Cells / metabolism
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Phylogeny
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Ranidae
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Receptors, Calcium-Sensing*
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Recoverin
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Rod Cell Outer Segment / chemistry*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Spectrometry, Fluorescence
Substances
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Antibodies, Monoclonal
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Calcium-Binding Proteins
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Calmodulin
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DNA Probes
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Eye Proteins
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Lipoproteins
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Nerve Tissue Proteins
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Neurocalcin
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Peptide Fragments
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RCVRN protein, human
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Rcvrn protein, mouse
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Receptors, Calcium-Sensing
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Recoverin
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Hippocalcin
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Nitric Oxide Synthase
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Amino Acid Oxidoreductases
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Guanylate Cyclase