Abstract
This paper identifies a neuronal receptor for tenascin-C (tenascin/cytotactin), an extracellular matrix protein that has previously been detected in developing sensory and motor neuron pathways and has been shown to regulate cell migration in the developing CNS. Antibodies specific for each subunit of the integrin alpha 8 beta 1 are used to demonstrate that alpha 8 beta 1 mediates neurite outgrowth of embryonic sensory and motor neurons on this extracellular matrix protein. In addition, expression of alpha 8 in K562 cells results in surface expression of alpha 8 beta 1 heterodimers that are shown to promote attachment of this cell line to tenascin. The major domain in tenascin that mediates neurite outgrowth is shown to be localized to fibronectin type III repeats 6-8.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cell Adhesion Molecules, Neuronal / metabolism*
-
Cell Adhesion Molecules, Neuronal / pharmacology
-
Cells, Cultured
-
Chick Embryo
-
Extracellular Matrix / metabolism
-
Extracellular Matrix Proteins / metabolism*
-
Extracellular Matrix Proteins / pharmacology
-
Fibronectins / chemistry
-
Ganglia, Spinal / ultrastructure
-
Immunosorbent Techniques
-
Integrin alpha Chains*
-
Integrins / analysis
-
Integrins / chemistry
-
Macromolecular Substances
-
Motor Neurons / metabolism*
-
Motor Neurons / ultrastructure
-
Neurites / physiology
-
Neurites / ultrastructure
-
Neurons, Afferent / metabolism*
-
Neurons, Afferent / ultrastructure
-
Receptors, Antigen / analysis
-
Receptors, Antigen / physiology*
-
Repetitive Sequences, Nucleic Acid
-
Tenascin
Substances
-
Cell Adhesion Molecules, Neuronal
-
Extracellular Matrix Proteins
-
Fibronectins
-
Integrin alpha Chains
-
Integrins
-
Macromolecular Substances
-
Receptors, Antigen
-
Tenascin
-
Tn receptor
-
integrin alpha8