Abstract
A polyclonal antibody against myosin light chain kinase (MLCK) of chicken gizzard recognized a 130 kd peptide of bullfrog sympathetic ganglia as MLCK. MLCK immunoreactivity was confined to the neuronal cell body. A synthetic peptide corresponding to an inhibitory domain of MLCK (Ala783-Gly804) was applied intracellularly to isolated sympathetic neurons during whole-cell recordings of ionic currents. The peptide inhibitor reversibly decreased M-type potassium current (IM) while not affecting A-type of delayed rectifier-type potassium currents. Intracellular application of an active fragment of MLCK enhanced IM, whereas application of an inactive MLCK fragment did not. The results suggest that IM can be modulated by MLCK-catalyzed phosphorylation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Barium / pharmacology
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Blotting, Western
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Chickens
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Ganglia, Sympathetic / physiology*
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Gizzard, Avian / enzymology
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Immunohistochemistry
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Membrane Potentials / drug effects
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Molecular Sequence Data
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Muscarine / pharmacology
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Muscle, Smooth / enzymology
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Myosin-Light-Chain Kinase / analysis
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Myosin-Light-Chain Kinase / metabolism*
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Neurons / drug effects
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Neurons / enzymology
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Neurons / physiology*
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Peptide Fragments / chemical synthesis
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Peptide Fragments / pharmacology
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Peptides / chemical synthesis
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Peptides / pharmacology*
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Potassium Channels / drug effects
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Potassium Channels / physiology*
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Rana catesbeiana
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Tyrosine 3-Monooxygenase / analysis
Substances
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Peptide Fragments
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Peptides
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Potassium Channels
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Barium
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Muscarine
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Tyrosine 3-Monooxygenase
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Myosin-Light-Chain Kinase