Light and electron microscopic immunocytochemistry was used to examine the serotonin (5-HT) innervation of the rat spinal trigeminal subnucleus caudalis. 5-HT-immunoreactive fibres form a dense plexus in lamina I and outer lamina II and synapse with the cell bodies and proximal dendrites of local neurones. Light microscopic double labelling revealed that the 5-HT axons contact enkephalin immunoreactive neurones in both laminae. The 5-fHT electron microscopic results indicate that at least some of these contacts are likely to be synapses. 5-HT axons are therefore in a position to exert a direct action on enkephalinergic interneurones and this may contribute to the analgesic actions of the 5-HT system.