It is feasible that small temporal variations in steady-state accommodation may provide feedback to the accommodation control system through changes in retinal image contrast and that this feedback may be used to maintain an optimal accommodation response. The complex waveform of microfluctuations is dominated by two distinct regions of activity; a low frequency component (LFC < 0.6 Hz) and a high frequency component (1.0 < or = HFC < or = 2.3 Hz). Whereas the HFCs appear to be correlated with some intraocular manifestation of arterial pulse the contribution of the LFCs to the control of steady-state accommodation is unclear. The present study investigates the effect of target luminance on the waveform of accommodative microfluctuations. Three young emmetropic observers viewed monocularly a high contrast (90%) Maltese cross target placed at a vergence equal to their dark-focus level of accommodation in a Badal stimulus system. The luminance of the target was varied from 0.002 to 11.63 cd m-2 in nine equal logarithmic steps. Five continuous accommodation signals were collected for each viewing condition at a sampling rate of 102.4 Hz, and average power spectra subsequently calculated with a frequency resolution of 0.1 Hz. One-way ANOVA revealed a significant variation in the root-mean-square (r.m.s.) value of the microfluctuations (F = 19.795, d.f. 124, P = 0.0001) which could be attributed mainly to increases in the r.m.s. value for the two lowest luminances (0.002 and 0.004 cd m-2). Power spectrum analysis revealed that these changes in the microfluctuations could be attributed to increases of power in the LFC.(ABSTRACT TRUNCATED AT 250 WORDS)