Abstract
Effects of the 'chemical phosphatase' 2,3-butanedione monoxide (BDM) on voltage-activated Ca2+ currents of adult rat superior cervical ganglion neurons were investigated using the whole-cell patch-clamp technique. BDM produced a rapid (< 10 s), reversible and dose-dependent (IC50 = 18.3 mM) inhibition of Ca2+ currents. The action of BDM was not prevented by 1 mM 8-(4-chlorophenylthio)-cAMP or 50 microM isoproterenol. H-7, a non-specific protein kinase inhibitor at 200 microM, did not prevent the rapid recovery from BDM-induced inhibition. Our results suggest that BDM inhibition of Ca2+ currents in rat sympathetic neurons does not require a 'chemical phosphatase' activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Animals
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Calcium / physiology*
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Calcium Channel Blockers / pharmacology*
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Cyclic AMP / analogs & derivatives
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Cyclic AMP / pharmacology
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Diacetyl / analogs & derivatives*
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Diacetyl / pharmacology
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Electrophysiology
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Ganglia, Sympathetic / cytology
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Ganglia, Sympathetic / physiology*
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Isoproterenol / pharmacology
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Isoquinolines / pharmacology
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Male
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Neurons / physiology*
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Phosphoric Monoester Hydrolases / metabolism*
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Piperazines / pharmacology
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Protein Kinase Inhibitors
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Rats
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Rats, Wistar
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Thionucleotides / pharmacology
Substances
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Calcium Channel Blockers
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Isoquinolines
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Piperazines
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Protein Kinase Inhibitors
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Thionucleotides
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diacetylmonoxime
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8-((4-chlorophenyl)thio)cyclic-3',5'-AMP
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Cyclic AMP
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Phosphoric Monoester Hydrolases
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Diacetyl
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Isoproterenol
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Calcium