Abstract
Chicken myosin-V is a member of a recently recognized class of myosins distinct from both the myosins-I and the myosins-II. We report here the purification, electron microscopic visualization, and motor properties of a protein of this class. Myosin-V molecules consist of two heads attached to an approximately 30 nm stalk that ends in a globular region of unknown function. Myosin-V binds to and decorates F-actin, has actin-activated magnesium-ATPase activity, and is a barbed-end-directed motor capable of moving actin filaments at rates of up to 400 nm/s. Myosin-V does not form filaments. Each myosin-V heavy chain is associated with approximately four calmodulin light chains as well as two less abundant proteins of 23 and 17 kd.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actins / metabolism
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Animals
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Brain / metabolism*
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Calmodulin / metabolism
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Calmodulin-Binding Proteins / isolation & purification
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Calmodulin-Binding Proteins / metabolism*
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Calmodulin-Binding Proteins / ultrastructure
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Chickens
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Egtazic Acid / pharmacology
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Electrophoresis, Polyacrylamide Gel
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Freeze Etching
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Macromolecular Substances
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Microscopy, Electron
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Models, Structural
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Myosin Type V*
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Nerve Tissue Proteins / isolation & purification
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Nerve Tissue Proteins / metabolism*
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Nerve Tissue Proteins / ultrastructure
Substances
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Actins
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Calmodulin
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Calmodulin-Binding Proteins
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Macromolecular Substances
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Nerve Tissue Proteins
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chicken brain myosin-V p190
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Egtazic Acid
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Myosin Type V