Abstract
Repulsive guidance cues can steer neuronal growth cones during development and prevent mature axons from regenerating. We have identified a 100 kd glycoprotein in the chick brain that is a good candidate for a repulsive cue. Since it induces the collapse and paralysis of neuronal growth cones in vitro, we have named it collapsin. It is effective at concentrations of approximately 10 pM. The C-terminal half of collapsin contains a single immunoglobulin-like domain and an additional highly basic region. The N-terminal half of collapsin shares significant homology with fasciclin IV, a growth cone guidance protein in grasshopper. Recombinant collapsin causes sensory ganglion growth cones to collapse but not retinal ganglion cell growth cones. We propose that collapsin could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain Chemistry*
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Cells, Cultured
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Chickens
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Cloning, Molecular
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DNA, Complementary / genetics
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Ganglia / growth & development
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Ganglia, Sensory / growth & development
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Glycoproteins / genetics
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Glycoproteins / isolation & purification
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Glycoproteins / pharmacology*
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Molecular Sequence Data
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Neurons / physiology*
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Organ Culture Techniques
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Organ Specificity
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / pharmacology
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Retina / growth & development
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Semaphorin-3A
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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Glycoproteins
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Recombinant Proteins
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Semaphorin-3A
Associated data
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GENBANK/L20888
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GENBANK/L20889
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GENBANK/L26083
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GENBANK/L33709
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GENBANK/S60904
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GENBANK/S60905
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GENBANK/S60924
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GENBANK/S66168
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GENBANK/U00968
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GENBANK/U02528