Asymmetry of the thalamofugal visual projections of the chick has been previously reported to exist in males but not females. Using a larger sample size and a double-labelling procedure, projections to the visual Wulst were labelled with the retrograde tracers True Blue and Fluoro-gold and the ratio of contralateral projections to ipsilateral projections was calculated. We found that asymmetry does exist in female chicks, but to a lesser degree than in males.