The amino-terminal tryptic peptide of bovine rhodopsin. A glycopeptide containing two sites of oligosaccharide attachment

Biochim Biophys Acta. 1977 May 27;492(1):83-94. doi: 10.1016/0005-2795(77)90216-1.

Abstract

A glycopeptide (T1) composed of 16 amino acids has been isolated from a tryptic digest of bovine rhodopsin. Its sequence is Met-Asn(CHO)-Gly-Thr-Glu-Gly-Pro-Asn-Phe-Tyr-Val-Pro-Phe-Ser-Asn(CHO)-Lys. Both rhodopsin and peptide T1 are blocked at their amino terminals. When a method specific for isolating amino-terminal tryptic peptides from proteins is applied to rhodopsin, peptide T1 is demonstrated to be the amino-terminal peptide of rhodopsin. Peptide T1 contains two sites at which carbohydrate is attached, whereas rhodopsin was previously thought to contain only a single such site.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Cattle
  • Glycopeptides / analysis*
  • Peptide Fragments / analysis
  • Retinal Pigments*
  • Rhodopsin*
  • Trypsin

Substances

  • Amino Acids
  • Glycopeptides
  • Peptide Fragments
  • Retinal Pigments
  • Rhodopsin
  • Trypsin