Abstract
Silencing is an epigenetic form of transcriptional regulation whereby genes are heritably, but not necessarily permanently, inactivated. We have identified the Saccharomyces cerevisiae genes SAS2 and SAS3 through a screen for enhancers of sir1 epigenetic silencing defects. SAS2, SAS3 and a Schizosaccharomyces pombe homologue are closely related to several human genes, including one associated with acute myeloid leukaemia arising from the recurrent translocation t(8;16)(p11;p13) and one implicated in HIV-1 Tat interactions. All of these genes encode proteins with an atypical zinc finger and well-conserved similarities to acetyltransferases. Sequence similarities and yeast mutant phenotypes suggest that SAS-like genes function in transcriptional regulation and cell-cycle exit and reveal novel connections between transcriptional silencing and human disease.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / genetics*
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Acute Disease
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Fungal
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Enhancer Elements, Genetic
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal*
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Gene Products, tat / genetics
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HIV-1 / genetics
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Humans
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Leukemia, Myeloid / genetics
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Molecular Sequence Data
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Mutation
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Phenotype
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Resting Phase, Cell Cycle
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Schizosaccharomyces / genetics
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Sequence Homology, Amino Acid
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Silent Information Regulator Proteins, Saccharomyces cerevisiae*
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Trans-Activators / genetics
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Zinc Fingers / genetics*
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tat Gene Products, Human Immunodeficiency Virus
Substances
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DNA, Fungal
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Fungal Proteins
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Gene Products, tat
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SIR1 protein, S cerevisiae
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Silent Information Regulator Proteins, Saccharomyces cerevisiae
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Trans-Activators
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tat Gene Products, Human Immunodeficiency Virus
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Acetyltransferases
Associated data
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GENBANK/L25608
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GENBANK/M11621
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GENBANK/M95912
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GENBANK/U14548
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GENBANK/U32686
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GENBANK/U33335
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GENBANK/U40989
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GENBANK/U47321
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GENBANK/U47742
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GENBANK/X06117
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GENBANK/X68628
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GENBANK/Z23261
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GENBANK/Z69795