Abstract
Sensory epithelia from normal rat utricles and those cultured with and without neomycin treatment were assayed for the presence of growth factor receptor mRNAs by RT-PCR (reverse transcriptase-polymerase chain reaction). Both undamaged and damaged utricles showed mRNA for Insulin receptor, IGF-I receptor, FGF receptor 1, EGF receptor, and PDGF alpha receptor. Neomycin-damaged sensory epithelia showed less PDGF alpha receptor mRNA than undamaged epithelia, suggesting that this message by expressed at higher copy levels in hair cells than in supporting cells. Consistent with that hypothesis, immunohistochemistry revealed much stronger PDGF alpha receptor staining in the hair cells than in the supporting cells. Preliminary evidence suggests that IGF-I receptor message also may be lowered in neomycin-damaged epithelia.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Anti-Bacterial Agents / toxicity
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Base Sequence
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Chickens
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Epithelium / drug effects
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Epithelium / injuries
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ErbB Receptors / genetics
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ErbB Receptors / metabolism
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Female
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Hair Cells, Auditory / drug effects
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Hair Cells, Auditory / injuries
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Hair Cells, Auditory / metabolism*
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Immunohistochemistry
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Molecular Sequence Data
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Neomycin / toxicity
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Oligonucleotide Probes
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Organ Culture Techniques
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Polymerase Chain Reaction
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Receptor, IGF Type 1 / genetics
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Receptor, IGF Type 1 / metabolism
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Receptor, Insulin / genetics
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Receptor, Insulin / metabolism
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Receptor, Platelet-Derived Growth Factor alpha
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Receptors, Fibroblast Growth Factor / genetics
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Receptors, Fibroblast Growth Factor / metabolism
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Receptors, Platelet-Derived Growth Factor / genetics*
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Receptors, Platelet-Derived Growth Factor / metabolism
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Saccule and Utricle / drug effects
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Saccule and Utricle / injuries*
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Saccule and Utricle / metabolism
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Tissue Fixation
Substances
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Anti-Bacterial Agents
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Oligonucleotide Probes
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RNA, Messenger
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Receptors, Fibroblast Growth Factor
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ErbB Receptors
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Receptor, IGF Type 1
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Receptor, Insulin
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Receptor, Platelet-Derived Growth Factor alpha
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Receptors, Platelet-Derived Growth Factor
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Neomycin