The receptor tyrosine kinase, Cek8, is transiently expressed on subtypes of motoneurons in the spinal cord during development

K Ohta; M Nakamura; K Hirokawa; S Tanaka; A Iwama; T Suda; M Ando; H Tanaka

doi:10.1016/0925-4773(95)00461-0

The receptor tyrosine kinase, Cek8, is transiently expressed on subtypes of motoneurons in the spinal cord during development

Mech Dev. 1996 Jan;54(1):59-69. doi: 10.1016/0925-4773(95)00461-0.

Authors

K Ohta¹, M Nakamura, K Hirokawa, S Tanaka, A Iwama, T Suda, M Ando, H Tanaka

Affiliation

¹ Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.

PMID: 8808406
DOI: 10.1016/0925-4773(95)00461-0

Abstract

Receptor tyrosine kinases (RTKs) play important roles in cellular proliferation, differentiation, and survival. We performed reverse transcriptase-polymerase chain reactions (RT-PCR) from enriched embryonic day 5 (E5) chick motoneurons by panning to identify RTKs involved in the early development of motoneuron. In situ hybridization revealed that Cek8, a member of the eph family, was specifically expressed on motoneurons at the brachial and lumbar segments of the spinal cord which innervate limb muscles, and disappeared after the naturally occurring cell death period (E6-E11). Immunohistochemistry using an anti-Cek8 monoclonal antibody showed the localization of Cek8 protein at the cell bodies and axonal fibers of motoneurons and muscles. The unique expression of Cek8 suggests its involvement in cellular survival or cell-cell interactions for specific subpopulations of developing motoneurons.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Apoptosis
Base Sequence
Cell Line
Cell Line, Transformed
Chick Embryo
Chlorocebus aethiops
Cloning, Molecular
DNA, Complementary / genetics
Enzyme Induction
Extremities / embryology
Extremities / innervation
Gene Expression Regulation, Developmental*
Humans
In Situ Hybridization
Kidney
Molecular Sequence Data
Motor Neurons / enzymology*
Nerve Tissue Proteins / biosynthesis*
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / immunology
Nerve Tissue Proteins / physiology
Neuromuscular Junction / enzymology
Neuropeptides / biosynthesis*
Neuropeptides / genetics
Neuropeptides / immunology
Neuropeptides / physiology
Polymerase Chain Reaction
Receptor Protein-Tyrosine Kinases / biosynthesis*
Receptor Protein-Tyrosine Kinases / genetics
Receptor Protein-Tyrosine Kinases / immunology
Receptor Protein-Tyrosine Kinases / physiology
Receptor, EphA4
Sequence Alignment
Spinal Cord / cytology
Spinal Cord / embryology*
Spinal Cord / enzymology
Transfection

Substances

Antibodies, Monoclonal
DNA, Complementary
Nerve Tissue Proteins
Neuropeptides
Receptor Protein-Tyrosine Kinases
Receptor, EphA4

Associated data

GENBANK/D38174