Abstract
We have cloned from human retina a cyclic nucleotide-gated (CNG) ion channel that is distinct from the one found in rod photoreceptors. This channel protein is highly homologous to the CNG channel that recently has been cloned from bovine testis and kidney and has been shown to be present in retinal cone photoreceptors. When expressed in human embryonic kidney cells, the protein forms functional ion channels with properties broadly similar to those described for the cloned bovine channel. The gene for this channel resides on chromosome 2.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cattle
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Cell Line
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Chromosome Mapping
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Chromosomes, Human, Pair 2*
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Cloning, Molecular
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Cyclic AMP / pharmacology
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Cyclic GMP / pharmacology
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Cyclic Nucleotide-Gated Cation Channels
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DNA Primers
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Electrophysiology
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Embryo, Mammalian
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Gene Expression
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Humans
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Ion Channels / chemistry
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Ion Channels / genetics*
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Ion Channels / physiology*
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Kidney
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Membrane Potentials / drug effects
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Molecular Sequence Data
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Polymerase Chain Reaction
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Retina / metabolism*
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Retinal Cone Photoreceptor Cells / metabolism*
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Sequence Homology, Amino Acid
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Transfection
Substances
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Cyclic Nucleotide-Gated Cation Channels
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DNA Primers
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Ion Channels
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Recombinant Proteins
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Cyclic AMP
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Cyclic GMP