Abstract
A dopaminergic neuronal cell line was established which stably expressed human Bcl-2 (MN9D/Bcl-2) or a control vector (MN9D/Neo). Overexpression of Bcl-2 cells led to robust neurite formation without cessation of cell division. Most markers of neuronal and/or dopaminergic maturation were unchanged although the synaptosomal associated protein, SNAP-25, was increased three-fold in MN9D/Bcl-2 cell lines. These data support a role for Bcl-2 in developmental processes at least during a certain stage(s) of neurogenesis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Blotting, Western
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Brain Neoplasms / enzymology
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Brain Neoplasms / metabolism
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Cell Line
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Dopamine / physiology*
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Humans
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Mice
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Neurites / physiology*
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Neurites / ultrastructure
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Neuroblastoma / enzymology
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Neuroblastoma / metabolism
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Neurons / enzymology
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Neurons / metabolism*
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Neurons / ultrastructure*
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-bcl-2
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Transfection
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Tumor Cells, Cultured
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Tyrosine 3-Monooxygenase / biosynthesis
Substances
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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Tyrosine 3-Monooxygenase
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Dopamine