A cyclic nucleotide-dependent chloride conductance in olfactory receptor neurons

J Membr Biol. 1997 Sep 1;159(1):53-60. doi: 10.1007/s002329900268.

Abstract

Whole-cell membrane currents were recorded from olfactory receptor neurons from the neotenic salamander Necturus maculosus. Cyclic nucleotides, released intracellularly by flash photolysis of NPE-caged cAMP or NPE-caged cGMP, activated a transient chloride current. The chloride current could be elicited at constant voltage in the absence of extracellular Ca2+ as well as in the presence of 3 mM intracellular Ca2+, suggesting that the current did not require either voltage or Ca2+ transients for activation. The current could be elicited in the presence of the protein kinase inhibitors H-7 and H-89, and in the absence of intracellular ATP, indicating that activation was independent of protein kinase A activity. These results suggest that Necturus olfactory receptor neurons contain a novel chloride ion channel that may be directly gated by cyclic nucleotides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
  • Adenosine Triphosphate / pharmacology
  • Animals
  • Calcium / metabolism*
  • Calcium / pharmacology
  • Chloride Channels / drug effects
  • Chloride Channels / physiology*
  • Cyclic AMP / analogs & derivatives
  • Cyclic AMP / pharmacology*
  • Cyclic AMP-Dependent Protein Kinases / antagonists & inhibitors
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Cyclic GMP / analogs & derivatives
  • Cyclic GMP / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • In Vitro Techniques
  • Isoquinolines / pharmacology
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Necturus
  • Olfactory Receptor Neurons / physiology*
  • Patch-Clamp Techniques
  • Photolysis
  • Sulfonamides*

Substances

  • Chloride Channels
  • Enzyme Inhibitors
  • Isoquinolines
  • Sulfonamides
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • Adenosine Triphosphate
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • Cyclic GMP
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • Calcium