Ras-GRF, the activator of Ras, is expressed preferentially in mature neurons of the central nervous system

R Zippel; N Gnesutta; N Matus-Leibovitch; E Mancinelli; D Saya; Z Vogel; E Sturani

doi:10.1016/s0169-328x(97)00120-4

Ras-GRF, the activator of Ras, is expressed preferentially in mature neurons of the central nervous system

Brain Res Mol Brain Res. 1997 Aug;48(1):140-4. doi: 10.1016/s0169-328x(97)00120-4.

Authors

R Zippel¹, N Gnesutta, N Matus-Leibovitch, E Mancinelli, D Saya, Z Vogel, E Sturani

Affiliation

¹ Department of General Physiology and Biochemistry, University of Milan, Italy.

PMID: 9379834
DOI: 10.1016/s0169-328x(97)00120-4

Abstract

In rodents, the Ras-specific guanine-nucleotide exchange factor (Ras-GRF) is expressed in different areas of the brain and, at a reduced level, also in the spinal cord. No expression of the 140 kDa Ras-GRF was detected in dorsal root ganglia and all other tissues tested. Analysis of primary cultures derived from brain reveals that this exchange factor is only present in neurons of the central nervous system. In primary hippocampal cultures, the expression of Ras-GRF increases in parallel with the onset of a neuronal network and in the whole brain it increases sharply after birth.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes / cytology
Astrocytes / metabolism
Brain / cytology
Brain / metabolism*
Calmodulin / analysis
Cells, Cultured
Embryo, Mammalian
Ganglia, Spinal / cytology
Ganglia, Spinal / metabolism
Guanine Nucleotide Exchange Factors
Neuroglia / cytology
Neuroglia / metabolism
Neurons / cytology
Neurons / metabolism*
Organ Specificity
Protein Biosynthesis*
Proteins / analysis
Rats
Rats, Wistar
Spinal Cord / cytology
Spinal Cord / metabolism*
ras Guanine Nucleotide Exchange Factors
ras-GRF1

Substances

Calmodulin
Guanine Nucleotide Exchange Factors
Proteins
ras Guanine Nucleotide Exchange Factors
ras-GRF1