Abstract
We have investigated the subunit composition and the molecular structure of the CNG channel expressed in rat pineal gland. Three types of subunits have been cloned: an alpha-subunit (CNG1), two beta-subunit splice variants (rCNG4.1 and rCNG4.2) and a hydrophilic glutamic acid-rich protein (rGARP). In situ hybridization with sections of rat brain revealed the co-expression of CNG1, CNG4 and GARP in pinealocytes. In addition, CNG4-specific transcripts were detected in the arcuate, periventricular and supraoptic nuclei of the hypothalamus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Brain / metabolism*
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Cloning, Molecular
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Codon
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Cyclic Nucleotide-Gated Cation Channels
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Humans
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Ion Channels / biosynthesis*
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Ion Channels / chemistry
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Macromolecular Substances
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Membrane Proteins / biosynthesis*
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Membrane Proteins / chemistry
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Molecular Sequence Data
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Organ Specificity
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Pineal Gland / metabolism*
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Rats
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription, Genetic*
Substances
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Codon
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Cyclic Nucleotide-Gated Cation Channels
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Ion Channels
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LRRC32 protein, human
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Macromolecular Substances
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Membrane Proteins
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rGarp protein, rat