Molecular cloning of cyclic nucleotide-gated cation channel subunits from rat pineal gland

Brain Res Mol Brain Res. 1997 Aug;48(1):171-5. doi: 10.1016/s0169-328x(97)00155-1.

Abstract

We have investigated the subunit composition and the molecular structure of the CNG channel expressed in rat pineal gland. Three types of subunits have been cloned: an alpha-subunit (CNG1), two beta-subunit splice variants (rCNG4.1 and rCNG4.2) and a hydrophilic glutamic acid-rich protein (rGARP). In situ hybridization with sections of rat brain revealed the co-expression of CNG1, CNG4 and GARP in pinealocytes. In addition, CNG4-specific transcripts were detected in the arcuate, periventricular and supraoptic nuclei of the hypothalamus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Brain / metabolism*
  • Cloning, Molecular
  • Codon
  • Cyclic Nucleotide-Gated Cation Channels
  • Humans
  • Ion Channels / biosynthesis*
  • Ion Channels / chemistry
  • Macromolecular Substances
  • Membrane Proteins / biosynthesis*
  • Membrane Proteins / chemistry
  • Molecular Sequence Data
  • Organ Specificity
  • Pineal Gland / metabolism*
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic*

Substances

  • Codon
  • Cyclic Nucleotide-Gated Cation Channels
  • Ion Channels
  • LRRC32 protein, human
  • Macromolecular Substances
  • Membrane Proteins
  • rGarp protein, rat