Brain-derived neurotrophic factor stimulates phosphorylation of stathmin in cortical neurons

J R Cardinaux; P J Magistretti; J L Martin

doi:10.1016/s0169-328x(97)00241-6

Brain-derived neurotrophic factor stimulates phosphorylation of stathmin in cortical neurons

Brain Res Mol Brain Res. 1997 Nov;51(1-2):220-8. doi: 10.1016/s0169-328x(97)00241-6.

Authors

J R Cardinaux¹, P J Magistretti, J L Martin

Affiliation

¹ Institut de Physiologie et Service de Neurologie du CHUV, Faculté de Médecine, Université de Lausanne, Switzerland.

PMID: 9427524
DOI: 10.1016/s0169-328x(97)00241-6

Abstract

We have identified by two-dimensional polyacrylamide gel electrophoresis a protein known as stathmin which is phosphorylated in a time- and concentration-dependent manner in response to brain-derived neurotrophic factor (BDNF) in primary cultures of cortical neurons. We show that stathmin phosphorylation is preceded by the activation of mitogen-activated protein kinase (MAPK) isoforms p44 and p42. Moreover, the MAPK kinase inhibitor PD 098059, which inhibits MAPK activation, also markedly reduces BDNF-stimulated phosphorylation of stathmin, therefore suggesting that phosphorylation of stathmin is triggered by the activation of MAPK. Phosphorylation of stathmin is specific for BDNF since nerve growth factor does not stimulate MAPK and stathmin phosphorylation in cultured cortical neurons. Taken together, these results identify stathmin as a new target protein of BDNF, possibly involved in the development of cortical neurons.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain-Derived Neurotrophic Factor / pharmacology*
Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
Cells, Cultured
Cerebral Cortex / metabolism*
Embryo, Mammalian
Enzyme Activation
Enzyme Inhibitors / pharmacology
Flavonoids / pharmacology
Kinetics
Mice
Microtubule Proteins*
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 1 / metabolism*
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases*
Neurons / drug effects
Neurons / metabolism*
Phosphates / metabolism
Phosphoproteins / drug effects
Phosphoproteins / metabolism*
Phosphorylation
Stathmin

Substances

Brain-Derived Neurotrophic Factor
Enzyme Inhibitors
Flavonoids
Microtubule Proteins
Phosphates
Phosphoproteins
Stathmin
Stmn1 protein, mouse
Calcium-Calmodulin-Dependent Protein Kinases
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one