The median preoptic nucleus is recognized as an important forebrain site involved in hydromineral and cardiovascular homeostasis. In the present study, whole cell patch-clamp recordings in parasagittal slices of adult rat brain were used to obtain information on the properties of median preoptic neurons. Lucifer Yellow-labelled cells demonstrated small ovoid somata with two to three aspiny main dendrites and axons that branched sparingly. Median preoptic neurons displayed varying degrees of hyperpolarization-activated time-dependent and/or time-independent inward rectification, and 86% of cells demonstrated low threshold spikes. Median preoptic nucleus is known to receive a prominent noradrenergic innervation from the medulla, and 59% of 156 tested neurons were found to respond to bath applied noradrenaline (1-100 microM). In the majority (n = 62) of cells, the response was an alpha 2 adrenoreceptor-mediated, tetrodotoxin-resistant, membrane hyperpolarization that was associated with a 43 +/- 6% increase in membrane conductance. The net noradrenaline-induced current (5-45 pA) was inwardly rectifying, cesium-resistant but barium sensitive. Current reversal at -102 +/- 4 mV in 3.1 mM [K]o and -62 +/- 3 mV in 10 mM [K]o implied opening of potassium channels. By contrast, a minority (n = 27) of cells responded to noradrenaline with an alpha 1-mediated, tetrodotoxin-resistant membrane depolarization. These observations imply a functional diversity among median preoptic neurons, and the prevalence of hyperpolarizing alpha 2 and, to a lesser extent, depolarizing alpha 1 adrenoreceptors on median preoptic neurons suggests that noradrenergic inputs can exert a prominent influence on their cellular excitability.