Abstract
We tested the model that neurons and epithelial cells use a shared mechanism for polarized protein sorting by comparing the pathways for localizing basolateral and postsynaptic proteins in C. elegans. GLR-1 glutamate receptors are localized to postsynaptic elements of central synapses and, when ectopically expressed, to basolateral membranes of epithelial cells. Proper localization of GLR-1 in both neurons and epithelia requires the PDZ protein LIN-10, defining LIN-10 as a shared component of the basolateral and postsynaptic localization pathways. Changing the GLR-1 carboxy-terminal sequence from a group I PDZ-binding consensus (-TAV) to a group II consensus (-FYV) restores GLR-1 synaptic localization in lin-10 mutants. Thus, these interneurons utilize at least two separate postsynaptic localization pathways.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Genetically Modified
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans Proteins*
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Cell Polarity / physiology
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Epithelial Cells / chemistry
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Epithelial Cells / cytology*
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Epithelial Cells / physiology
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Genes, Reporter
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Green Fluorescent Proteins
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Helminth Proteins / genetics*
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Helminth Proteins / metabolism*
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Indicators and Reagents
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Interneurons / chemistry
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Interneurons / cytology*
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Interneurons / physiology
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Luminescent Proteins / genetics
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Membrane Proteins / genetics
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Proteins*
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Synapses / chemistry
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Synaptic Vesicles / chemistry
Substances
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Caenorhabditis elegans Proteins
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Helminth Proteins
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Indicators and Reagents
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Luminescent Proteins
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Membrane Proteins
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Proteins
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lin-10 protein, C elegans
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Green Fluorescent Proteins