Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosis

B Zhang; Y H Koh; R B Beckstead; V Budnik; B Ganetzky; H J Bellen

doi:10.1016/s0896-6273(00)80664-9

Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosis

Neuron. 1998 Dec;21(6):1465-75. doi: 10.1016/s0896-6273(00)80664-9.

Authors

B Zhang¹, Y H Koh, R B Beckstead, V Budnik, B Ganetzky, H J Bellen

Affiliation

¹ Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA. bxz@bcm.tmc.edu

PMID: 9883738
DOI: 10.1016/s0896-6273(00)80664-9

Abstract

Clathrin-mediated endocytosis is thought to involve the activity of the clathrin adaptor protein AP180. However, the role of this protein in endocytosis in vivo remains unknown. Here, we show that a mutation that eliminates an AP180 homolog (LAP) in Drosophila severely impairs the efficiency of synaptic vesicle endocytosis and alters the normal localization of clathrin in nerve terminals. Most importantly, the size of both synaptic vesicles and quanta is significantly increased in lap mutants. These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adaptor Proteins, Vesicular Transport
Amino Acid Sequence
Animals
Axons / physiology
Axons / ultrastructure
CCAAT-Enhancer-Binding Proteins
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Drosophila / embryology
Drosophila / genetics
Embryo, Nonmammalian / physiology
Endocytosis*
Gene Expression Regulation, Developmental
Gene Library
Molecular Sequence Data
Monomeric Clathrin Assembly Proteins*
Nerve Endings / physiology
Nerve Endings / ultrastructure
Nerve Tissue Proteins / chemistry
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / metabolism
Nuclear Proteins / chemistry
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Phosphoproteins / chemistry
Phosphoproteins / genetics*
Phosphoproteins / metabolism
Sequence Alignment
Synaptic Vesicles / physiology*
Synaptic Vesicles / ultrastructure*

Substances

Adaptor Proteins, Vesicular Transport
CCAAT-Enhancer-Binding Proteins
DNA-Binding Proteins
Monomeric Clathrin Assembly Proteins
Nerve Tissue Proteins
Nuclear Proteins
Phosphoproteins
clathrin assembly protein AP180

Associated data

GENBANK/AF075247
GENBANK/Q61548
GENBANK/U45976
GENBANK/U88308