… to confirm primer specificity and the comparative C t (threshold cycle) method was for
quantification according to the following formula: C t = C t (target gene) − C t (reference gene). The …

Functional muscle fiber denervation is a major contributor to the decline in physical function
observed with aging and is now a recognized cause of sarcopenia, a muscle disorder …

… The brains were removed from the skull, post-fixed in 4% PFA for 2 h at 4C, and then … to
48 h at 4C. Then, the brains were frozen on dry ice and stored at – 70C until further processing. …

… Panels C and D show time courses of averaged changes in fEPSP slope induced by the θ-burst
stimulation in the absence (light circles) or in the presence (dark circles) of BDNF in …

… Maximum temperatures of 50 deg to 51 deg C were observed during a period of 7 days
lasting 2h 52min. day-1 in average at a depth of 10 cm. Temperatures between 40-45 deg C …

Proper cerebral function depends on appropriate production and positioning of neurons,
formation of adequate neuronal connectivity and requires stringent balance of excitatory and …

… a McIlwain tissue chopper - Figure 10 (C) - and allowed to recover, … Completed RT reactions
were stored at −20 ◦C until use. … *Aliquots of stock solutions were kept frozen at –20º C until …

Objective Rett syndrome is the main cause of intellectual disability in females and is caused
mainly by mutations in the X-linked MECP2 gene. In Rett syndrome, brain-derived …

Pathogen genome sequencing during epidemics enhances our ability to identify and understand
suspected clusters and investigate their relationships. Here, we combine genomic and …

… non-homologous end-joining (c-NHEJ and alt-NHEJ) and single-strand annealing (SSA)
DNA repair [60]. Most notably, efficiency of c-NHEJ is reduced following transfection with any of …