‘The development of a multicellular organism from a single egg cell requires both the
determination of many cell types and the organization of these cells into an elaborate pattern (…

The success of Drosophila melanogaster as a model organism is largely due to the power
of forward genetic screens to identify the genes that are involved in a biological process. …

… However, since single staufen domains do not show sequencespecific binding in vitro (St
Johnston et al., 1992), it still remains to be proven that staufen binds directly to either the oskar …

… stages and Northern blot analysis were performed as described in St Johnston et al. (1990),
and the cDNA library was screened following the protocol of Brown and Kafatos (1988). …

We have identified a double-stranded (ds)RNA-binding domain in each of two proteins: the
product of the Drosophila gene staufen, which is required for the localization of maternal …

mRNA localization is a common mechanism for targeting proteins to regions of the cell
where they are required. It has an essential role in localizing cytoplasmic determinants, …

… D We could observe particles clearly only during interphase 14. In this case, the RNA
behaves similarly to the … was performed as in St Johnston et ai. (1991) and in situ hybridization …

Anterior–posterior polarity in Drosophila arises from the movement of the oocyte to the
posterior of the egg chamber, and the subsequent acquisition of posterior fate by the adjacent …

Cell polarity, the generation of cellular asymmetries, is necessary for diverse processes in
animal cells, such as cell migration, asymmetric cell division, epithelial barrier function, and …

… Grünert and D.St Johnston, unpublished results). In contrast, interfacial residues from
helix α1 are highly conserved for each domain when different species are examined, but are …